Viruses, Vol. 16, Pages 669: Identification of HLA-A*11:01 and A*02:01-Restricted EBV Peptides Using HLA Peptidomics

Viruses, Vol. 16, Pages 669: Identification of HLA-A*11:01 and A*02:01-Restricted EBV Peptides Using HLA Peptidomics Viruses doi: 10.3390/v16050669 Authors: Yufei Wang Wanlin Zhang Ruona Shi Yanran Luo Zhenhuan Feng Yanhong Chen Qiuting Zhang Yan Zhou Jingtong Liang Xiaoping Ye Qisheng Feng Xiaofei Zhang Miao Xu Epstein-Barr Virus (EBV) is closely linked to nasopharyngeal carcinoma (NPC), notably prevalent in southern China. Although type II latency of EBV plays a crucial role in the development of NPC, some lytic genes and intermittent reactivation are also critical for viral propagation and tumor progression. Since T cell-mediated immunity is effective in targeted killing of EBV-positive cells, it is important to identify EBV-derived peptides presented by highly prevalent human leukocyte antigen class I (HLA-I) molecules throughout the EBV life cycle. Here, we constructed an EBV-positive NPC cell model to evaluate the presentation of EBV lytic phase peptides on streptavidin-tagged specific HLA-I molecules. Utilizing a mass spectrometry (LC-MS/MS)-based immunopeptidomic approach, we characterized eleven novel EBV peptides as well as two previously identified peptides. Furthermore, we determined these peptides were immunogenic and could stimulate PBMCs from EBV VCA/NA-IgA positive donors in an NPC endemic southern Chinese population. Overall, this work demonstrates that highly prevalent HLA-I-specific EBV peptides can be captured and functional...
Source: Viruses - Category: Virology Authors: Tags: Article Source Type: research