Sensors, Vol. 24, Pages 2643: Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes

Sensors, Vol. 24, Pages 2643: Electrochemical Impedance Spectroscopy for the Sensing of the Kinetic Parameters of Engineered Enzymes Sensors doi: 10.3390/s24082643 Authors: Adriána Dusíková Timea Baranová Ján Krahulec Olívia Dakošová Ján Híveš Monika Naumowicz Miroslav Gál The study presents a promising approach to enzymatic kinetics using Electrochemical Impedance Spectroscopy (EIS) to assess fundamental parameters of modified enteropeptidases. Traditional methods for determining these parameters, while effective, often lack versatility and convenience, especially under varying environmental conditions. The use of EIS provides a novel approach that overcomes these limitations. The enteropeptidase underwent genetic modification through the introduction of single amino acid modifications to assess their effect on enzyme kinetics. However, according to the one-sample t-test results, the difference between the engineered enzymes and hEKL was not statistically significant by conventional criteria. The kinetic parameters were analyzed using fluorescence spectroscopy and EIS, which was found to be an effective tool for the real-time measurement of enzyme kinetics. The results obtained through EIS were not significantly different from those obtained through traditional fluorescence spectroscopy methods (p value >> 0.05). The study validates the use of EIS for measuring enzyme kinetics and provides insight into the effects of spec...
Source: Sensors - Category: Biotechnology Authors: Tags: Article Source Type: research