Freshly Prepared Graphene Oxide Nanoparticles ’ Wound-Healing Potential and Antibacterial Activity Specifically Against Staphylococcus aureus: In Vivo Efficacy and Clinical Isolate Evaluation

AbstractGraphene oxide (GO) exhibits several characteristics worthy of biomedical applications. The current study aimed to synthesize graphene oxide nanoparticles (GONPs) for antibacterial and wound-healing efficacy evaluations. The GONPs were prepared using Hummer ’s method and characterized by FT-IR, UV-Vis, Raman, SEM spectroscopic, and electron micrograph-based analyses, respectively, to confirm the structure and morphology. The analyses revealed GONPs as flaky, nano-sheeted structures with irregular shapes and sizes that were stacked on top of each othe r. The clinical isolates of gram-negative, i.e.,Escherichia coli,Klebsiella pneumoniae, andPseudomonas aeruginosa, as well as gram-positive, i.e.,Staphylococcus aureus, microbes were incubated for 24 h at 37 ℃ with 62.5, 125, and 250 µg/ml concentrations of the freshly prepared GONPs which showed significant antimicrobial activity. All the pathogens were comparatively more active to 250 µg/ml concentration of GONPs with inhibition zone diameters reaching 17.06, 15.10, 31.00, and 18.36 mm, respective ly, as compared with the 250 µg/ml concentration of cephalexin. TheS. aureus–infected mouse wound-infection model utilized for in vivo investigation showed healing, when treated, twice daily, for 15 days, with GONPs at 250 and 500 mg/kg doses. The wound’s contraction was observed to assess the therapeutic efficacy of the GONPs. The average wound healing of the treated g roups was higher than that of the control. The...
Source: Plasmonics - Category: Biomedical Science Source Type: research