KSCBi005-A-10(hiPSC-HIF1alphaKO), a HIF1alpha knockout human induced pluripotent stem cell line, for demonstrating the role of cellular response to hypoxia

Stem Cell Res. 2024 Apr 6;77:103415. doi: 10.1016/j.scr.2024.103415. Online ahead of print.ABSTRACTUnder hypoxia, hypoxia-inducible factor (HIF)-1 regulates hypoxia-inducible genes, such as vascular endothelial growth factor (VEGF) and its receptors VEGFR1 and VEGFR2. It is an oxygen-dependent transcriptional activator that plays a crucial role in tumor angiogenesis and mammalian embryo development. It is a heterodimeric protein comprising a constitutively expressed HIF-1β subunit and the highly regulated HIF-1α subunits. Using CRISPR-Cas9 genome editing, we generated biallelic HIF-1α mutants in human induced pluripotent stem cells (hiPSCs). The HIF-1α homozygous-knockout hiPSCs retained their normal morphology, gene expression, and in vivo differentiation potential.PMID:38636267 | DOI:10.1016/j.scr.2024.103415
Source: Cell Research - Category: Cytology Authors: Source Type: research