Diagnostic investigation of Avian Reovirus Field Variants Circulating in Broiler Chickens in Pennsylvania of United States between 2017-2022

This report provides our diagnostic investigation on monitoring ARV field variants from broiler chickens in Pennsylvania. Genomic characterization findings of 72 ARV field isolates obtained from broiler cases during the last six years indicated that the six distinct cluster variant strains (genotype I-VI) continuously circulated in PA poultry, which were genetically diverse and distant from the vaccine and vaccine-related field strains. Most of the variants clustered within genotype V (24/72, 33.3%), followed by genotype II (16/72, 22.2%), genotype IV (13/72, 18.1%), genotype III (13/72, 18.1%), genotype VI (05/72, 6.94%), and genotype I (1/72, 1.38%). The amino acid identity between 72 field variants and the vaccine strains (1133, 1733, 2408, 2177) varied from 45.3% to 99.7%, while the difference in amino acid counts ranged from 1 - 164. Among the field variants, the amino acid identity and count difference ranged from 43.3% to 100% and 0 to 170, respectively. Variants within genotype V had maximum amino acid identity (94.7-100%), whereas none of the variants within genotypes II and VI were alike. These findings indicate the continuing occurrence of multiple ARV genotypes in the environment.PMID:38629680 | DOI:10.1080/03079457.2024.2342889
Source: Avian Pathology - Category: Pathology Authors: Source Type: research