GSE261920 Human long noncoding RNA, VILMIR, is induced by major respiratory viral infections and modulates the host interferon response

Contributors : Kristen John ; Ian Huntress ; Ethan Smith ; Hsuan Chou ; Tammy S Tollison ; Sergio Covarrubias ; Elisa Crisci ; Susan Carpenter ; Xinxia PengSeries Type : Non-coding RNA profiling by high throughput sequencingOrganism : Homo sapiensLong noncoding RNAs (lncRNAs) are a newer class of noncoding transcripts identified as key regulators of biological processes. Here we aimed to identify novel lncRNA targets that play critical roles in major human respiratory viral infections by systematically mining large-scale transcriptomic datasets. Using bulk RNA-sequencing (RNA-seq) analysis, we identified a previously uncharacterized lncRNA, named virus inducible lncRNA modulator of interferon response (VILMIR), that was consistently upregulated after in vitro influenza infection across multiple human epithelial cell lines and influenza A virus subtypes. VILMIR was also upregulated after SARS-CoV-2 and RSV infections in vitro. We experimentally confirmed the response of VILMIR to influenza infection and interferon-beta (IFN- β) treatment in the A549 human epithelial cell line and found the expression of VILMIR was robustly induced by IFN-β treatment in a dose and time-specific manner. Single cell RNA-seq analysis of bronchoalveolar lavage fluid (BALF) samples from COVID-19 patients uncovered that VILMIR was upregulate d across various cell types including at least five immune cells. The upregulation of VILMIR in immune cells was further confirmed in the human T cell ...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Non-coding RNA profiling by high throughput sequencing Homo sapiens Source Type: research