Neuron-microelectrode junction induced by an engineered synapse organizer

In this study, we engineered a downsized variant of the artificial synapse organizer based on neurexin1β and a peptide-tag, fabricated gold microelectrodes functionalized with the receptor for the organizer, and performed validation experiments in primary cultured neurons. Successful inductions of synapse-like junctions were detected at the sites of contact between neurons expressing the engineered synapse organizer and functionalized microelectrodes, but not in the negative control experiment in which the electrode functionalization was omitted. Such a molecularly inducible neuron-microelectrode junction could be the basis for the next-generation electrophysiological technique enabling cell type-selective recording.PMID:38626529 | DOI:10.1016/j.bbrc.2024.149935
Source: Biochemical and Biophysical Research communications - Category: Biochemistry Authors: Source Type: research