Cancers, Vol. 16, Pages 1482: Patient-Derived Exosomes as siRNA Carriers in Ovarian Cancer Treatment

Cancers, Vol. 16, Pages 1482: Patient-Derived Exosomes as siRNA Carriers in Ovarian Cancer Treatment Cancers doi: 10.3390/cancers16081482 Authors: Aasa Shimizu Kenjiro Sawada Masaki Kobayashi Yukako Oi Tadashi Oride Yasuto Kinose Michiko Kodama Kae Hashimoto Tadashi Kimura RNA interference is a powerful gene-silencing tool with potential clinical applications. However, its therapeutic use is challenging because suitable carriers are unavailable. Exosomes are stable small endogenous vesicles that can transport functional molecules to target cells, making them ideal small interfering RNA (siRNA) carriers. Herein, we elucidated the therapeutic potential of patient-derived exosomes as an siRNA carrier for ovarian cancer (OC) treatment. The exosomes were extracted from the culture medium of primary fibroblasts collected from the omentum of patients with OC during surgery. MET proto-oncogene, receptor tyrosine kinase (MET) was selected for gene silencing, c-Met siRNAs were synthesized and loaded into the exosomes (Met-siExosomes) via electroporation, and the treatment effect of the Met-siExosomes was assessed in vitro and in vivo. The Met-siExosomes downregulated the c-Met protein levels and inhibited OC cell proliferation, migration, and invasion. In xenograft experiments using SKOV3-13 and ES-2 cells, Met-siExosomes were selectively extracted from peritoneally disseminated tumors. Intraperitoneal treatment suppressed the c-Met downstream targets in cancer ...
Source: Cancers - Category: Cancer & Oncology Authors: Tags: Article Source Type: research