Investigating Circadian Gating of Temperature Responsive Genes

Methods Mol Biol. 2024;2795:213-225. doi: 10.1007/978-1-0716-3814-9_20.ABSTRACTUnderstanding gene expression dynamics in the context of the time of day and temperature response is an important part of understanding plant thermotolerance in a changing climate. Performing "gating" experiments under constant conditions and light-dark cycles allows users to identify and dissect the contribution of the time of day and circadian clock to the dynamic nature of stress-responsive genes. Here, we describe the design of specific laboratory experiments in plants (Arabidopsis thaliana and bread wheat, Triticum aestivum) to investigate temporal responses to heat (1 h at 37 °C) or cold (3 h at 4 °C), and we include known marker genes that have circadian-gated responses to temperature changes.PMID:38594541 | DOI:10.1007/978-1-0716-3814-9_20

Source: Mol Biol Cell - April 9, 2024 Category: Molecular Biology Authors: Rachel I Strout Calum A Graham Antony N Dodd Dawn H Nagel Source Type: research