Genes, Vol. 15, Pages 464: Different Nuclear Architecture in Human Sperm According to Their Morphology

Genes, Vol. 15, Pages 464: Different Nuclear Architecture in Human Sperm According to Their Morphology Genes doi: 10.3390/genes15040464 Authors: Nino-Guy Cassuto Nesrine Ogal Said Assou Lea Ruoso Eli-Jonathan Rogers Miguel-José Monteiro Daniel Thomas Jean-Pierre Siffroi Alexandre Rouen Human sperm parameters serve as a first step in diagnosing male infertility, but not in determining the potential for successful pregnancy during assisted reproductive technologies (ARTs) procedures. Here, we investigated the relationship between sperm head morphology at high magnification, based on strict morphologic criteria, and the nuclear architecture analyzed by fluorescence in situ hybridization (FISH). We included five men. Two of them had an elevated high-magnification morphology score of 6 points (Score 6) indicating high fertility potential, whereas three had a low score of 0 points (Score 0), indicating low fertility potential. We used FISH to study the inter-telomeric distance and the chromosomal territory area of chromosome 1 (Chr. 1). We then compared these two parameters between subjects with high and low scores. FISH data analysis showed that the inter-telomeric distance (ITD) and chromosomal territory area (CTA) of Chr. 1 were significantly higher in subjects with low scores (score 0) than high scores (score 6). Our results suggest that (i) there is a link between nuclear architecture and sperm head abnormalities, particularly vacuoles; and (ii) it is ...
Source: Genes - Category: Genetics & Stem Cells Authors: Tags: Brief Report Source Type: research