An integrated multi-omics analysis reveals osteokines involved in global regulation
In this study, we analyzed bone and extraskeletal tissues, osteoblast (OB) conditioned media, bone marrow supernatant (BMS), and serum, for basal osteokines and those responsive to aging and mechanical loading/unloading. We identified 375 candidate osteokines and their changes in response to aging and mechanical dynamics by integrating data from RNA-seq, scRNA-seq, and proteomic approaches. Furthermore, we analyzed their cellular sources in the bone and inter-organ communication facilitated by them (bone-brain, liver, and aorta). Notably, we discovered that senescent OBs secrete fatty-acid-binding protein 3 to propagate senescence toward vascular smooth muscle cells (VSMCs). Taken together, we identified previously unknown candidate osteokines and established a dynamic regulatory network among them, thus providing valuable resources to further investigate their systemic roles.PMID:38574738 | DOI:10.1016/j.cmet.2024.03.006
Source: Cell Metabolism - Category: Cytology Authors: Wenquan Liang Tiantian Wei Le Hu Meijun Chen Liping Tong Wu Zhou Xingwei Duan Xiaoyang Zhao Weijie Zhou Qing Jiang Guozhi Xiao Weiguo Zou Di Chen Zhipeng Zou Xiaochun Bai Source Type: research