C166 EVs potentiate miR cardiac reprogramming via miR-148a-3p

We have demonstrated that directly reprogramming cardiac fibroblasts into new cardiomyocytes via miR combo improves cardiac function in the infarcted heart. However, major challenges exist with delivery and efficacy. During a screening based approach to improve delivery, we discovered that C166-derived EVs were effective delivery agents for miR combo both in vitro and in vivo. In the latter, EV mediated delivery of miR combo induced significant conversion of cardiac fibroblasts into cardiomyocytes (~20%), reduced fibrosis and improved cardiac function in a myocardial infarction injury model.
Source: Journal of Molecular and Cellular Cardiology - Category: Cytology Authors: Source Type: research