Global transcriptome analysis reveals Salmonella Typhimurium employs nitrate metabolism to combat bile  stress

This study identifies transcriptional responses in bile-tolerant WT and bile-sensitive ΔcspE. Upregulation of several genes involved in nitrate metabolism was observed, includingfnr, a global regulator of nitrate metabolism. Notably, Δfnr was susceptible to bile stress. Also, complementation withfnr lowered reactive oxygen species and enhanced the survival of bile-sensitive ΔcspE. Importantly, intracellular nitrite amounts were highly induced in bile-treated WT compared to ΔcspE. Also, the WT strain pre-treated with nitrate displayed better growth with bile. These results demonstrate that nitrate-dependent metabolism promotes adaptation ofS.  Typhimurium to bile.
Source: FEBS Letters - Category: Biochemistry Authors: Tags: Research Article Source Type: research