Simple and Rapid Detection of ESBL bla < sub > SHV < /sub > gene from an Urban River in Tokyo by Loop-Mediated Isothermal Amplification

Biocontrol Sci. 2022;27(4):209-215. doi: 10.4265/bio.27.209.ABSTRACTExtended-spectrum β-lactamases (ESBLs) are produced mainly by gram-negative bacteria in Enterobacteriaceae. One of the major types of ESBLs is sulfhydryl variable (SHV) -type ESBL. Herein, we attempted to develop a simple and rapid method for the detection of the ESBL blaSHV gene by loop-mediated isothermal amplification (LAMP) . The five-primer set designed could amplify blaSHV gene at an isothermal temperature of 65℃. The detection limit of the LAMP method with the LF loop primer was 1 copy/tube, which was 10,000-fold more sensitive than that of the conventional PCR. The LAMP assay could also detect the direct amplification of blaSHV gene from a single river water sample in Tokyo. The LAMP method has great potential for applications in hospital, soil and water environment, food, and livestock.PMID:36567117 | DOI:10.4265/bio.27.209

Source: Biocontrol Science - December 25, 2022 Category: Microbiology Authors: Masahiko Okai Rintaro Endo Masachika Takashio Masami Ishida Naoto Urano Source Type: research