Effect of < em > Gracilaria changii < /em > extract and its fraction in reverse cholesterol transport by targeting < em > SR-BI < /em > gene

Nat Prod Res. 2024 Mar 14:1-8. doi: 10.1080/14786419.2024.2327851. Online ahead of print.ABSTRACTThis present study aimed to investigate the effects of Gracilaria changii (Red seaweed) extract and its fraction on scavenger receptor class B type I (SR-BI) gene expressions. In vitro studies of the activity of G. changii extract and its fraction against the therapeutic target for hypocholesterolemia were conducted using high-density lipoprotein (HDL) receptor SR-BI via luciferase assay in HepG2 cells. In the current study, methanol crude extract (MCE) and Fraction-2 of G. changii showed the highest expression levels of the SR-BI gene via luciferase assay and increased SR-BI mRNA expression compared to the negative control. Metabolite profiling of the MCE of G. changii by GC-MS revealed nine major compounds, comprising various fatty acids, particularly hexadecenoic acid. Therefore, the MCE and Fraction-2 of G. changii have the potential to be explored in the treatment of hypercholesterolaemia to prevent or reduce the severity of atherosclerosis development.PMID:38486409 | DOI:10.1080/14786419.2024.2327851
Source: Natural Product Research - Category: Biochemistry Authors: Source Type: research