Bioburden detection on surface and water samples in a rapid, ultra ‐sensitive and high‐throughput manner
The objective of this study is to develop and validate an assay and protocol for detecting bioburden on solid surfaces, as well as in water, with high sensitivity and accuracy in a rapid manner. Henceforth, a resazurin-based assay optimized for detecting bioburden has been integrated with a previously developed portable multichannel fluorometer. The microbes were isolated from solid surfaces in different laboratory settings by swabbing technique, and stream water was collected for contamination analysis. Based on the results, the assay and protocol can successfully detect bioburden as low as 20 CFU/cm2 and 10 CFU/mL present in both surface and water samples, respectively.
Source: Biotechnology Progress - Category: Biotechnology Authors: Md Sadique Hasan,
Chad Sundberg,
Elias Gilotte,
Xudong Ge,
Yordan Kostov,
Govind Rao Tags: RESEARCH ARTICLE Source Type: research
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