Lack of Syndecan-1 promotes the pathogenesis of experimental rheumatoid arthritis

This study explores the involvement level of Sdc-1 in the development of RA in a collagen II –induced arthritis mice model. RA was induced in two mice strains (wild-type BALB/c group and Sdc-1 knockout) by collagen II. Mice underwent regular clinical observations and scoring. After sacrifice, leg biopsies were taken from mice for histological examination, using a variety of stains. In add ition, proteins were extracted, and molecular assessment of TNF-α was performed using the western blot technique. In the Sdc-1 knockout group, clinical scoring results showed a significantly more severe experimental RA; histology showed a significant increase in bone erosion, cartilage destruction, inflammation, and less granulated mast cells than the wild-type. In addition, molecular assessment of TNF-α showed more increase in expression in the Sdc-1 knockout models compared to the wild-type. Data suggest that lack of Sdc-1 enhances the inflammatory characteristics in RA. However, more mole cular studies and investigations are needed to determine its exact role and possible mechanisms involved.
Source: Immunogenetics - Category: Genetics & Stem Cells Source Type: research