Genes, Vol. 15, Pages 365: Characterization and Functional Analysis of Fads Reveals & Delta;5 Desaturation Activity during Long-Chain Polyunsaturated Fatty Acid Biosynthesis in Dwarf Surf Clam Mulinia lateralis
Genes, Vol. 15, Pages 365: Characterization and Functional Analysis of Fads Reveals Δ5 Desaturation Activity during Long-Chain Polyunsaturated Fatty Acid Biosynthesis in Dwarf Surf Clam Mulinia lateralis
Genes doi: 10.3390/genes15030365
Authors:
Tianhao Teng
Zhenghua Zheng
Wenqian Jiao
Na Liu
Ao Wang
Mengjiao Liu
Le Xie
Zujing Yang
Jingjie Hu
Zhenmin Bao
Fatty acid desaturases (Fads), as key enzymes in the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFAs), catalyze the desaturation between defined carbons of fatty acyl chains and control the degree of unsaturation of fatty acids. In the present study, two Fads genes, designated MulFadsA and MulFadsB, were identified from the genome of the dwarf surf clam Mulinia lateralis (Mollusca, Mactridae), and their spatiotemporal expression was examined. MulFadsA and MulFadsB contained the corresponding conserved functional domains and clustered closely with their respective orthologs from other mollusks. Both genes were expressed in the developmental stages and all tested adult tissues of M. lateralis, with MulFadsA exhibiting significantly higher expression levels in adult tissues than MulFadsB. Subsequently, the effects of dietary microalgae on Fads expressions in the dwarf surf clam were investigated by feeding clams with two types of unialgal diets varying in fatty acid content, i.e., Chlorella pyrenoidosa (Cp) and Platymonas helgolandica (Ph). The results show that the expression...
Source: Genes - Category: Genetics & Stem Cells Authors: Tianhao Teng Zhenghua Zheng Wenqian Jiao Na Liu Ao Wang Mengjiao Liu Le Xie Zujing Yang Jingjie Hu Zhenmin Bao Tags: Article Source Type: research