Effects of a bitter substance, denatonium benzoate, on pancreatic hormone secretion

This study investigated the effect of the bitter substance, denatonium benzoate (DB), on hormone secretion from mouse pancreatic islets and INS-1 832/13 cells. DB (0.5-1mM) augmented insulin secretion at both 2.8mM and 16.7mM glucose. This effect was no longer present at 5mM DB likely due to greater levels of cellular apoptosis. DB-stimulated insulin secretion involved closure of the KATP channel, activation of T2R signalling in beta-cells and intra-islet GLP-1 release. DB also enhanced glucagon and somatostatin secretion, but the underlying mechanism was less clear. Together, this study demonstrates that the bitter substance, DB, is a strong potentiator of islet hormone secretion independent of glucose. This observation highlights the potential for widespread off-target effects associated with the clinical use of bitter-tasting substances.PMID:38477876 | DOI:10.1152/ajpendo.00046.2024
Source: Am J Physiol Endocri... - Category: Endocrinology Authors: Source Type: research