Chemical Constituents from Agave applanata and Its Antihyperglycemic, Anti-inflammatory, and Antimicrobial Activities Associated with Its Tissue Repair Capability
In this study, the
chemical composition of this ethanol tonic is established and its association with
antihyperglycemic, anti-inflammatory, antimicrobial, and wound healing activities is
discussed. The fresh leaves of A. applanata were extracted with
ethanol : H2O (85 : 15). A fraction of this extract was lyophilized, and the
remainder was partitioned into CH2Cl2, n-BuOH, and water.
CH2Cl2 and n-BuOH fractions were subjected to a successive
open column chromatography process. The structure of the isolated compounds was
established using nuclear magnetic resonance and mass spectrometry spectra. The
antihyperglycemic activity was evaluated through in vivo sucrose and glucose
tolerance experiments, as well as ex vivo intestinal absorption and hepatic
production of glucose. Wound healing and edema inhibition were assayed in mice. The
minimum inhibitory concentrations (MICs) of the hydroalcoholic extract, its fractions, and
pure compounds were determined through agar microdilution against the most isolated
pathogens from diabetic foot ulcers. Fatty acids, β-sitosterol, stigmasterol,
hecogenin (1), N-oleyl-D-glucosamine, β-daucosterol, sucrose,
myo-inositol, and
hecogenin-3-O-α-L-rhamnopyranosyl-(1 → 3)-β-D-xylopyranosyl-(1 → 2)-[β-D-xylopyranosyl-(1 → 3)-β-D-glucopyranosyl-(1 → 3)]-β-D-glucopyranosyl-(1 → 4)-β-D-galactopyranoside
(2) were charact...
Source: Planta Medica - Category: Drugs & Pharmacology Authors: Aguilar-Guadarrama, A. Berenice D íaz-Román, Mónica Aideé Osorio-Garc ía, Maribel D éciga-Campos, Myrna Rios, Mar ía Yolanda Tags: Original Papers Source Type: research
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