G ‐quadruplex is involved in the regulation of BmSGF1 expression in the Silkworm, Bombyx mori

In this study, aBmSGF1 G4−/− homozygous mutant was generated with the G4 sequence knocked out. The promoter activity ofBmSGF1 was lowered in theBmSGF1 G4−/− mutant. Pyridostatin (PDS) stabilized the G4 structure and increased the promoter activity ofBmSGF1, whereas anti-sense oligonucleotide (ASO) complementary to the G4 sequence suppressed the promoter activity ofBmSGF1. Compared with wild-type larvae, the deletion of theBmSGF1 G4 structure decreased both the expression ofBmSGF1 and the fibroin heavy chain geneBmFib-H in the posterior silk gland and the weight of the cocoons. Overall, these results suggest that the promoter G4 structure ofBmSGF1 participates in the transcription regulation of theBmSGF1 gene in the silkworm.

Source: Insect Science - March 5, 2024 Category: Biology Authors: Yanfei Chen, Jin Li, Kangkang Niu, Yuling Peng, Qisheng Song, Qili Feng Tags: ORIGINAL ARTICLE Source Type: research