Synergistic promotion of transient transgene expression in CHO cells by PDI/XBP-1s co-transfection and mild hypothermia

In conclusion, the combination of PDI/XBP-1s overexpression and culture temperature optimization can achieve higher transient expression of recombinant protein, which provides a synergetic strategy to improve transient production of recombinant protein in CHO cells.PMID:38416261 | DOI:10.1007/s00449-024-02987-5

Source: Bioprocess and Biosystems Engineering - February 28, 2024 Category: Biomedical Engineering Authors: Xi Zhang Yaokun Wang Dandan Yi Chi Zhang Binhuan Ning Yushun Fu Yanlong Jia Tianyun Wang Xiaoyin Wang Source Type: research