Discovery of a pimaradiene that decreases viability of MDA-MB-468 cells through inhibition of EGFR signaling pathway

Chem Biodivers. 2024 Feb 28:e202400288. doi: 10.1002/cbdv.202400288. Online ahead of print.ABSTRACTTriple-negative breast cancer (TNBC) is characterized by strong invasiveness, high relapse rates and poor overall survival and occurs in approximately 15-20% of all breast cancer cases. Natural compounds are a promising option for managing breast cancer. ent-8(14),15-Pimaradiene-2β,19-diol (JXE-23), is a pimaradiene isolated from the fern Aleuritopteris albofusca. However, the effects and molecular mechanisms of JXE-23 on cancer cells are still unknown. Thus, this study was designed to determine the potential of JXE-23 for its anticancer properties in TNBC cells. JXE-23 was evaluated for their antiproliferative activity in vitro against human breast cancer cell lines, and showed selectively cytotoxic activity against MDA-MB-468 EGFR-overexpressing TNBC cancer cell lines, with an IC50 value at 1.17±0.04 μM. Moreover, mechanistic investigations indicated that JXE-23 was significantly capable of inhibiting cell proliferation and viability of MDA-MB-468 cells. In addition, JXE-23 exerted an anticancer effect against MDA-MB-468 cells via restraining cell migration in a dose-dependent mode. Moreover, after treatment with JXE-23, the protein expressions of pEGFR, pERK, pAKT and p70S6K were significantly reduced in MDA-MB-468 cells. The results underscored that JXE-23 could be a potential lead compound for the treatment of EGFR-overexpressing TNBC cells.PMID:38415947 | DOI:10.1002/cb...
Source: Chemistry and Biodiversity - Category: Biochemistry Authors: Source Type: research