Evaluating Fourier-transform infrared spectroscopy with IR Biotyper as a faster and simpler method for investigating the sources of an outbreak of legionellosis

AbstractFourier-transform infrared (FTIR) spectroscopy using the IR Biotyper and core genome single  nucleotide polymorphism (cgSNP) analysis were performed on 12Legionella isolates associated with an outbreak at a spa house in Kanagawa Prefecture, Japan, and 3 non-outbreak isolates. The discriminative power of FTIR spectroscopy for 48-h incubation conditions ofL.pneumophila in this outbreak was lower than cgSNP-based typing but higher than serogroup typing. FTIR spectroscopy could screen outbreak isolates from a group of genetically related isolates and may be useful as an initial typing method inLegionella outbreak investigations.
Source: European Journal of Clinical Microbiology and Infectious Diseases - Category: Microbiology Source Type: research