Neuroprotective mechanism of ribisin A on H < sub > 2 < /sub > O < sub > 2 < /sub > -induced PC12 cell injury model

Tissue Cell. 2024 Feb 3;87:102322. doi: 10.1016/j.tice.2024.102322. Online ahead of print.ABSTRACTRibisin A has been shown to have neurotrophic activity. The aim of this study was to evaluate the neuroprotective effect of ribisin A on injured PC12 cells and elucidate its mechanism. In this project, PC12 cells were induced by H2O2 to establish an injury model. After treatment with ribisin A, the neuroprotective mechanism of ribisin A was investigated by methyl tetrazolium (MTT) assay, Enzyme-linked immunosorbent assay (ELISA), flow cytometric analysis, fluorescent probe analysis, and western blot. We found that ribisin A decreased the rate of lactate dehydrogenase (LDH) release, increased cellular superoxide dismutase (SOD) level, decreased the levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), Ca2+ expression and reactive oxygen species (ROS). Moreover, ribisin A significantly increased mitochondrial membrane potential (MMP) and inhibited apoptosis of PC12 cells. Meanwhile, ribisin A activated the phosphorylation of ERK1/2 and its downstream molecule CREB by upregulating the expression of Trk A and Trk B, the upstream molecules of the ERK signaling pathway.PMID:38367324 | DOI:10.1016/j.tice.2024.102322

Source: Tissue and Cell - February 17, 2024 Category: Cytology Authors: Xin Zhang Mengyu Bao Jingyi Zhang Lihao Zhu Di Wang Xin Liu Lingchuan Xu Lijuan Luan Yuguo Liu Yuhong Liu Source Type: research