Variation in the affinity of three representative avian adenoviruses for the cellular coxsackievirus and adenovirus receptor

In this study, we successfully solved the crysta l structure of the FAdV-4 fiber1 knob at 1.6 Å resolution. The interaction between the fibre knob and different domains of CAR was verified by confocal microscopy, coimmunoprecipitation and surface plasmon resonance (SPR) analysis. The fibre knobs of the three representative fowl adenoviruses speci fically recognized CAR domain 1 (D1), but the recognition of CAR domain 2 (D2) by chicken embryo lethal orphan (CELO) strains was weak. These results provide insights into the differences in adenovirus‒host cell interactions and have important implications for the exploration of viral invasion mec hanisms.
Source: Veterinary Research - Category: Veterinary Research Source Type: research