Soluble meso and deuteroporphyrin analogs of the malaria pigment hematin anhydride

J Inorg Biochem. 2024 Mar;252:112470. doi: 10.1016/j.jinorgbio.2023.112470. Epub 2023 Dec 22.ABSTRACTTwo soluble heme analogs of the insoluble malaria pigment hematin anhydride (HA, or β-hematin), [Fe(III)(protoporphyrin)]2, with either mesoporphyrin (MHA) or deuteroporphyrin (DHA) are characterized by elemental analysis, SEM, IR spectroscopy, electronic spectroscopy, paramagnetic 1H NMR spectroscopy and solution magnetic susceptibility. While prior single crystal and X-ray powder diffraction results indicate all three have a common propionate linked dimer motif, there is considerable solid state variation in the conformation. This is associated with enhanced solubility of MHA and DHA. As with HA, DHA undergoes thermally promoted reversible hydration/dehydration in the solid state. Solution 1H NMR studies of DHA suggest a high spin dimeric structure with the porphyrin methyls distributed between two isomers which are also present in the solid state. These soluble iron(III)porphyrin dimers allow for the first direct solution studies by NMR and UV-Vis spectroscopies of these key species. Taken together the results illustrate the importance and utility of varying the substituents on the periphery of the porphyrin for studying heme aggregation and malaria pigment formation.PMID:38218137 | DOI:10.1016/j.jinorgbio.2023.112470

Source: Journal of Inorganic Biochemistry - January 13, 2024 Category: Biochemistry Authors: Liliana Su árez Aaron J Kosar Erin L Dodd Dagobert Tazoo Ambre Charlotte Lambert D Scott Bohle Source Type: research