Exploring the dynamics and structure of PpiB in living Escherichia coli cells using electron paramagnetic resonance spectroscopy
We report a significant decrease in the dynamics induced by the cellular environment for two chosen labeling positions. These changes could not be reproduced by adding crowding agents and cell extracts. Concomitantly, we report a broadening of the distance distribution inE. coli, determined by Gd(III) –Gd(III) DEER measurements, as compared with solution and human HeLa cells. This suggests an increase in the number of PpiB conformations present inE. coli cells, possibly due to interactions with other cell components, which also contributes to the reduction in mobility and suggests the presence of a quinary structure.
Source: Protein Science - Category: Biochemistry Authors: Yasmin Ben ‐Ishay,
Yoav Barak,
Akiva Feintuch,
Olivier Ouari,
Annalisa Pierro,
Elisabetta Mileo,
Xun‐Cheng Su,
Daniella Goldfarb Tags: RESEARCH ARTICLE Source Type: research