Density-based lipoprotein depletion improves extracellular vesicle isolation and functional analysis

CONCLUSION: Combination of G-UC+SEC significantly reduced EV lipoprotein contamination without interfering in EV cellular source, gene ontology and ultrastructure, allowing the obtention of highly pure EVs with potential implications for functional assays, proteomic and lipidomic analysis.PMID:38278418 | DOI:10.1016/j.jtha.2024.01.010

Source: Thrombosis and Haemostasis - January 26, 2024 Category: Hematology Authors: Laura Botelho Merij Luana Rocha da Silva Lohanna Palhinha Milena Tavares Gomes Paula Ribeiro Braga Dib Remy Martins-Gon çalves Kemily Toledo-Quiroga Marcus Ant ônio Raposo-Nunes Fernanda Brandi Andrade Sharon de Toledo Martins Ana L úcia Rosa Nasciment Source Type: research