In Vivo Potency of Hematopoietic Stem Cells after Long-Term Storage at -80C in DMSO+HES Cryprotectant Mixture: Comparing Engraftment Kinetics and Efficacy Data in First and Second Autologous Stem Cell Transplantation in Myeloma

Two methods of hematopoietic stem cell (HSC) cryopreservation exist: 10% DMSO alone using rate-controlled freezing with storage in liquid nitrogen (LN2) and 5% DMSO/6%HES with ‘dump’ into and storage in mechanical freezers at -80C. Long-term storage at LN2 is clinically efficacious, but clinical data for long-term storage in DMSO/HES is lacking. To assess clinical benefit of HSC stored long term in DMSO+HES at -80C, we report on first and second autologous stem cell t ransplants (ASCT1 and 2) in patients undergoing sequential transplants for multiple myeloma (MM) using HSC stored>2 years.
Source: Biology of Blood and Marrow Transplantation - Category: Hematology Authors: Tags: 200 Source Type: research