Polymorphism in < em > IGFALS < /em > gene and its association with scrotal circumference in Hu lambs

In this study, the scrotal circumference of 1353 male Hu sheep at different stages of development was measured and descriptive statistical analysis was performed. The results showed that the coefficient of variation of scrotal circumference at each stage was greater than 10%, and its heritability were moderately to high, ranging from 0.318 to 0.719. We used PCR amplification and Sanger sequencing to scan the polymorphisms of the IGFALS gene, and performed association analysis with the circumference of the scrotum at different stages. We identified a synonymous mutation g.918 G > C in exon 1 of the IGFALS gene, and this mutation was significantly associated with scrotal circumference at 100, 120, 140, 160 and 180 days (p < 0.05). Therefore, IGFALS gene polymorphism can be used as a molecular marker affecting scrotal circumference of Hu sheep, which can provide a reference for future molecular marker-assisted selection of scrotal circumference in sheep.PMID:38174897 | DOI:10.1080/10495398.2023.2295928

Source: Animal Biotechnology - January 4, 2024 Category: Biotechnology Authors: Zongwu Ma Weimin Wang Deyin Zhang Xinji Wang Shirong Li Liming Zhao Yukun Zhang Yuan Zhao Xiaolong Li Changchun Lin Jianghui Wang Jiangbo Cheng Dan Xu Xiaobin Yang Yongliang Huang Panpan Cui Jia Liu Xiwen Zeng Rui Zhai Zhiqiang Huang Xiuxiu Weng Xiaoxue Z Source Type: research