Highly efficient genome editing via CRISPR-Cas9 ribonucleoprotein (RNP) delivery in mesenchymal stem cells

In this study, we compared and optimized plasmid DNA and RNP approaches for efficient genome engineering in MSCs. The RNP-mediated approach enabled genome editing with high indel frequency and low cytotoxicity in MSCs. By utilizing Cas9 RNPs, we successfully generated B2M-knockout MSCs, which reduced T-cell differentiation, and improved MSC survival. Furthermore, this approach enhanced the immunomodulatory effect of IFN-r priming. These findings indicate that the RNP-mediated engineering of MSC genomes can achieve high efficiency, and engineered MSCs offer potential as a promising therapeutic strategy.PMID:38053293

Source: BMB Reports - December 6, 2023 Category: Biochemistry Authors: A Reum Han Ha Rim Shin Jiyeon Kweon Soo Been Lee Sang Eun Lee Eun-Young Kim Eun-Ju Chang Yongsub Kim Seong Who Kim Source Type: research