Galectin from Trichinella spiralis alleviates DSS-induced colitis in mice by regulating the intestinal microbiota

AbstractAccording to numerous reports,Trichinella spiralis (T. spiralis) and its antigens can reduce intestinal inflammation by modulating regulatory immunological responses in the host to maintain immune homeostasis. Galectin has been identified as a protein that is produced byT. spiralis, and its characterization revealed this protein has possible immune regulatory activity. However, whether recombinantT. spiralis galectin (rTs-gal) can cure dextran sulfate sodium (DSS)-induced colitis remains unknown. Here, the ability ofrTs-gal to ameliorate experimental colitis in mice with inflammatory bowel disease (IBD) as well as the potential underlying mechanism were investigated. The disease activity index (DAI), colon shortening, inflammatory cell infiltration, and histological damage were used as indicators to monitor clinical symptoms of colitis. The results revealed that the administration ofrTs-gal ameliorated these symptoms. According to Western blotting and ELISA results,rTs-gal may suppress the excessive inflammatory response-mediated induction of TLR4, MyD88, and NF- κB expression in the colon. Mice with colitis exhibit disruptions in the gut flora, including an increase in gram-negative bacteria, which in turn can result in increased lipopolysaccharide (LPS) production. However, injection ofrTs-gal may inhibit changes in the gut microbiota, for example, by reducing the prevalence ofHelicobacter andBacteroides, which produce LPS. The findings of the present study reveale...
Source: Veterinary Research - Category: Veterinary Research Source Type: research