Anthraquinones from the roots of Morinda scabrida Craib exhibit antiproliferative activity against A549 lung cancer cells and antitubulin polymerization

Fitoterapia. 2023 Dec 19:105781. doi: 10.1016/j.fitote.2023.105781. Online ahead of print.ABSTRACTSix anthraquinones were isolated from Morinda scabrida Craib, an unexplored species of Morinda found in the tropical forest of Thailand. All six anthraquinones showed cytotoxicity against A549 lung cancer cells, with the most active compound, nordamnacanthal (MS01), exhibiting the IC50 value of 16.3 ± 2.5 μM. The cytotoxic effect was dose-dependent and led to cell morphological changes characteristic of apoptosis. In addition, flow cytometric analysis showed dose-dependent apoptosis induction and the G2/M phase cell cycle arrest, which was in agreement with the tubulin polymerization inhibitory activity of MS01. Molecular docking analysis illustrated the binding between MS01 and the α/β-tubulin heterodimer at the colchicine binding site, and UV-visible absorption spectroscopy revealed the DNA binding capacity of MS01.PMID:38128619 | DOI:10.1016/j.fitote.2023.105781

Source: Fitoterapia - December 21, 2023 Category: Biochemistry Authors: Sorachai Sae-Lim Lukana Ngiwsara Kriengsak Lirdprapamongkol Thamrongjet Puttamuk Sutida Maneeanakekul Poomsith Thangsan Withsakorn Sangsuwan Jisnuson Svasti Pitak Chuawong Source Type: research