Genomic and transcriptomic characteristics of 12 novel primary cell lines derived from three patients with cholangiocarcinoma
Genomics. 2023 Dec 17:110765. doi: 10.1016/j.ygeno.2023.110765. Online ahead of print.ABSTRACTCholangiocarcinoma (CCA) is an aggressive bile duct malignancy with poor prognosis. To improve our understanding of the biological characteristics of CCA and develop effective therapies, appropriate preclinical models are required. Here, we established and characterized 12 novel patient-derived primary cancer cell (PDPC) models using multi-region sampling. At the genomic level of PDPCs, we observed not only commonly mutated genes, such as TP53, JAK3, and KMT2C, consistent with the reports in CCA, but also specific mutation patterns in each cell line. In addition, specific expression patterns with distinct biological functions and pathways involved were also observed in the PDPCs at the transcriptomic level. Furthermore, the drug-sensitivity results revealed that the PDPCs exhibited different responses to the six commonly used compounds. Our findings indicate that the established PDPCs can serve as novel in vitro reliable models to provide a crucial molecular basis for improving the understanding of tumorigenesis and its treatment.PMID:38113975 | DOI:10.1016/j.ygeno.2023.110765
Source: Genomics - Category: Genetics & Stem Cells Authors: Feiling Feng Xianglei Xing Guojuan Jiang Xiaoya Xu Bin Li Zhizhen Li Zhenghua Xie Yue Wu Xiaofang Li Qingxiang Gao Dongyu Liu Yang Shen Jianyang Ao Dadong Zhang Yanxin Chang Xiaoqing Jiang Source Type: research
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