Multiomic profiling of transplant glomerulopathy reveals a novel T-cell dominant subclass

Kidney transplant (KTx) biopsies showing transplant glomerulopathy (TG) (glomerular basement membrane double contours (cg)> 0)) and microvascular inflammation (MVI) in the absence of C4d staining and donor-specific antibodies (DSA)s do not fulfill the criteria for chronic active antibody-mediated rejection (CA-AMR) diagnosis and do not fit into any other Banff category. To investigate this, we initiated a multicenter intercontinental study encompassing 36 cases, comparing the immunomic and transcriptomic profiles of 14 KTx biopsies classified as cg+MVI DSA-/C4d- with 22 classified as CA-AMR DSA+/C4d+ through novel transcriptomic analysis using the NanoString Banff-Human Organ Transplant (B-HOT) panel and subsequent orthogonal subset analysis using two innovative 5-marker multiplex immunofluorescent panels.

Source: Kidney International - December 19, 2023 Category: Urology & Nephrology Authors: Iacopo Cristoferi, Hilal Varol, Myrthe van Baardwijk, Layla Rahiem, Karishma A. Lila, Thierry P.P. van den Bosch, Carla C. Baan, Dennis A. Hesselink, Rafael Kramann, Robert C. Minnee, Dana A.M. Mustafa, Marlies E.J. Reinders, Dave L. Roelen, Shazia P. Sha Tags: clinical investigation Source Type: research