Secretome analysis: Reading cellular sign language to understand intercellular communication

Mol Cell Proteomics. 2023 Dec 9:100692. doi: 10.1016/j.mcpro.2023.100692. Online ahead of print.ABSTRACTA significant portion of mammalian proteomes is secreted to the extracellular space to fulfill crucial roles in cell-to-cell communication. To best recapitulate the intricate and multi-faceted crosstalk between cells in a live organism, there is an ever-increasing need for methods to study protein secretion in model systems that include multiple cell types. In addition, post-translational modifications further expand the complexity and versatility of cellular communication. This review aims to summarise recent strategies and model systems that employ cellular co-culture, chemical biology tools, protein enrichment and proteomic methods to characterize the composition and function of cellular secretomes. This is all geared towards gaining better understanding of organismal biology in vivo mediated by secretory signaling.PMID:38081362 | DOI:10.1016/j.mcpro.2023.100692
Source: Molecular and Cellular Proteomics : MCP - Category: Molecular Biology Authors: Source Type: research