Phage display-derived cross-reactive neutralizing antibody against enterovirus 71 and coxsackievirus A16.

Phage display-derived cross-reactive neutralizing antibody against enterovirus 71 and coxsackievirus A16.
Jpn J Infect Dis. 2015 Jun 12;
Authors: Zhang X, Sun C, Xiao X, Pang L, Shen S, Zhang J, Cen S, Yang BB, Huang Y, Sheng W, Zeng Y
Abstract
Enterovirus 71 (EV71) and coxsackievirus A16 (CVA16) are the members of the Picornaviridae family and considered as major causative agents of hand, foot and mouth disease (HFMD). Large outbreaks of HFMD caused by EV71 and CVA16 infection are becoming a substantial public health concern in the Asia-Pacific region in the past decades. No particular vaccine or antiviral drugs are yet available against EV71 and CVA16. In the current study, a chimeric antibody targeting a highly conserved peptide in EV71 VP4 protein was successfully isolated by using phage display technique, which showed cross-neutralizing ability against EV71 and CVA16 in vitro. The obtained data suggested a great potential for phage display-derived antibody to be a candidate of broad neutralizing antibody against EV71 and CVA16 after affinity maturation and humanization.
PMID: 26073737 [PubMed - as supplied by publisher]

Source: Japanese Journal of Infectious Diseases - June 12, 2015 Category: Infectious Diseases Authors: Zhang X, Sun C, Xiao X, Pang L, Shen S, Zhang J, Cen S, Yang BB, Huang Y, Sheng W, Zeng Y Tags: Jpn J Infect Dis Source Type: research