GSE221721 Transcriptomic analysis of resistant and wild-type Botrytis ci-nerea isolates revealed fludioxonil-resistant mechanisms

Contributors : Xuncheng Wang ; Mei Liu ; Liusheng DuanSeries Type : Expression profiling by high throughput sequencingOrganism : Botrytis cinereaBotrytis cinerea (gray mold) is one of the most destructive pathogens of cherry tomatoes, causing fruit decay and economic loss. Fludioxonil is an effective fungicide widely used for crop protec-tion and is essential for controlling tomato gray mold. The emergence of fungicide-resistant strains has made the control of Botrytis cinerea more difficult. While the genome of Botrytis cinerea is available, there are few reports regarding the large-scale functional annotation of the genome using expressed genes derived from transcriptomes, and the mechanism(s) underlying such flu-dioxonil resistance remain unclear. The present study prepared RNA-sequencing (RNA-seq) li-braries for three Botrytis cinerea strains [two highly resistant (LR and FR) versus one highly sen-sitive (S) to fludioxonil], with and without fludioxonil treatment, to identify fludioxonil responsive genes that facilitate fungicide resistance. Functional enrichment analysis identified nine resistant related DEGs in the fludioxonil-induced LR and FR transcriptome that were simultaneously up regulated, and seven resistant related DEGs down regulated. These included adenosine tri-phosphate (ATP)-binding cassette (ABC) transporter-encoding genes, major facilitator super-family (MFS) transporter-encoding genes, and the high-osmolarity glycerol (HOG) pathway homologues or rela...

Source: GEO: Gene Expression Omnibus - December 12, 2023 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Botrytis cinerea Source Type: research