Red propolis extract as a natural ionophore for confined sheep: performance and morphological and histopathological changes

This study aimed to evaluate the effect of increasing levels of red propolis extract (RPE) in the diet of confined sheep on performance and histomorphometric parameters of rumen and intestine and histopathological parameters of liver and kidney. Thirty-five male sheep (17.08 ± 2.36 kg) were used, distributed in a completely randomized design, with five treatments (0, 7, 14, 21, and 28 mL day-1 RPE) and seven replications, submitted to 68 days of experiment. At the end of the experimental period, the animals were euthanized, and samples of rumen, intestine, liver, and kidney were collected to histomorphometry and histopathology analyzes. Higher RPE inclusions (21 and 28 mL day-1) maintained dry matter intake and increased total weight (5.78x 6.14 and 6.95 kg, respectively) gain up to 20.24%. In the rumen, the inclusion of RPE led to an increase in the thickness of the epithelium and the highest level also increased the thickness of the keratinized portion of this epithelium (21.71x 32.15 μm). The level of 21 mL day-1 provided larger ruminal papillae (1620.68x 1641.70 μm) and greater ruminal absorption area (561791.43 x 698288.50 μm2). In intestine 21 and 28 mL-1 of RPE provided greater mucosal thickness (468.54x 556.20 and 534.64 μm), higher goblet cell index (23.32x 25.82 and 25.64) and higher hepatic glycogen index (1.47x 1.64 and 1.62), supporting higher nutrients absortion and glicogenolise and intestinal health, corroborating the weight gain indices. The inclusion of...
Source: Tropical Animal Health and Production - Category: Veterinary Research Source Type: research