Comparative genome analysis of colistin-resistant < i > Escherichia coli < /i > harboring < i > mcr < /i > isolated from rural community residents in Ecuador and Vietnam

by Hoa Thi Thanh Hoang, Mayumi Yamamoto, Manuel Calvopina, Carlos Bastidas-Caldes, Diep Thi Khong, Thang Nam Nguyen, Ryuji Kawahara, Takahiro Yamaguchi, Yoshimasa Yamamoto The spread of colistin-resistant bacteria among rural community residents of low- and middle-income countries is a major threat to community health. Although the mechanism of the spread of colistin-resistant bacteria in communities is unknown, geographic and regional characteristics may influence it. To elucidate the spread mechanism of colistin-resistant bacteria, we analyzed the genomes of colistin-resistantEscherichia coli isolated from Vietnam and Ecuador residents, which are geographically and socially different. Stool specimens of 139 and 98 healthy residents from Ecuador and Vietnam rural communities, respectively, were analyzed for colistin-resistantE.coli withmcr. Its prevalence in the residents of all the communities assessed was high and approximately equal in both countries: 71.8% in Ecuador and 69.4% in Vietnam. A phylogenetic tree analysis revealed that the sequence type of colistin-resistantE.coli was diverse and the major sequence types were different between the two countries. The location ofmcr in the isolates showed that the proportion of chromosomalmcr was 35.1% and 8.5% in the Vietnam and Ecuador isolates, respectively. Most of these chromosomalmcr genes (75% –76%) had an intactmcr-transposon Tn6330. Contrastingly, the replicon types of themcr-carrying-plasmids were diverse in both c...
Source: PLoS One - Category: Biomedical Science Authors: Source Type: research