Dynamic interactions between Candida albicans and different streptococcal species in a multispecies oral biofilm

This study aims to investigate the role of streptococcal species in the development of oral biofilm and the cross-kingdom interactions between some of the members of the commensal MSG and the pathogen yeastC. albicans using a multispecies supragingival biofilm model. A total of nine different in vitro biofilms were grown, quantified with culture analyses, and visually examined with confocal laser scanning microscopy (CLSM). A four-species biofilm without any streptococcal species was used as a basic biofilm. In each subsequent inoculum, one species of MSG was added and afterward combined withStreptococcus mutans. The eight-species biofilm contained all eight strains used in this study. Culture analyses showed that the presence ofS. mutans in a four-species biofilm withStreptococcus oralis orS. oralis subsp.tigurinus did not differ significantly inC. albicans colony-forming unit (CFU) counts compared to biofilms withoutS. mutans. However, compared to othermitis species,Streptococcus gordonii combined withS. mutans resulted in the lowest CFUs ofC. albicans. Visual observation by CLSM showed that biofilms containing bothS. mutans and one species of MSG seemed to induce the formation of filamentous form ofC. albicans. However, when several species of MSG were combined withS. mutans,C. albicans was again found in its yeast form.
Source: MicrobiologyOpen - Category: Microbiology Authors: Tags: ORIGINAL ARTICLE Source Type: research