GSE210497 SARS-CoV-2 pathogenesis mediated by E protein's PBM was prevented by modulators of CFTR function

Contributors : Jose M Honrubia ; Francisco J Guti érrez-Álvarez ; Alejandro Sanz ; Diego Muñoz ; Ezequiel González ; Carlos Castaño-Rodríguez ; Jorge Ripoll ; Ana Esteban ; Raúl Fernández-Delgado ; Juan C Oliveros ; Stanley Perlman ; Paul McCray ; Luis EnjuanesSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusThe three human deadly coronaviruses (CoVs) (SARS-CoV, MERS-CoV and SARS-CoV-2) have infected around eight thousand, two thousand six hundred and 262 million people so far (December 2021), causing the death of 10%, 37% and 2% of them, respectively, so their implication in health is very important. These CoVs have proteins with a PBM motif that binds to PDZ cell domains. PDZ domains are found in more than 400 cellular proteins, therefore, viruses with PBM motifs have a high potential to modify cell behavior. It was studied the implication of the E protein PBM motif of various virulent or attenuated human CoVs (hCoVs) in the pathogenesis induced by these viruses. Variants of SARS-CoV, MERS-CoV and SARS-CoV-2 that lack the E protein PBM have been generated by reverse genetics and their pathogenicity has been analyzed in mice. The PBM motifs of these three hCoVs have been shown to be virulence factors and participate in their replication. Furthermore, a collection of SARS-CoV mutants has been constructed in which the E protein PBM domain was replaced by the one derived from virulent or attenuated hCoVs, and their virulen...
Source: GEO: Gene Expression Omnibus - Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research