A novel primary porcine retinal pigment epithelium cell model with preserved properties

CONCLUSIONS: This work describes, for the first time, a pRPE cell model that exhibits preserved RPE properties for several passages on cell culture plastic plates. Though RPE characteristics were maintained for at least 6 passages, the reduced CRALBP and RPE-65 with passaging emphasize that lower passage cells are advantageous to utilise, and that morphology, barrier function and ZO-1 localisation cannot be solely employed as a quality measure of RPE identity. Pigs are phylogenetically similar to humans, including similar physiology, anatomy and immune system. Therefore, porcine RPE cells constitute a relevant model system for studying human eye diseases, such as AMD.PMID:37725007 | DOI:10.1080/02713683.2023.2259636
Source: Current Eye Research - Category: Opthalmology Authors: Source Type: research