Robust and bioaffinity-enhanced nanocarrier based immunochromatographic assay with simplified sample preparation for pentachlorophenol sodium in animal tissues

Talanta. 2023 Sep 9;267:125190. doi: 10.1016/j.talanta.2023.125190. Online ahead of print.ABSTRACTSample pretreatment is directly related to the accuracy and reproducibility of test results, simple and controllable sample pretreatment can greatly reduce analysis errors. Herein, MIL-88B-NH2 was synthesized with a regular octahedral rigid framework structure and used as antibody carrier to develop an immunochromatographic assay (ICA) for the detection of sodium pentachlorophenol (PCP) in animal tissues. MIL-88B-NH2 has superior molar absorption coefficient, ultrahigh antibody affinity, and extreme detection environmental tolerance, which were 1.57, 2.5, and 2 times higher than those of traditional colloidal gold, respectively. Therefore, even complex animal tissues can be detected by simple extraction without nitrogen blowing. Ultimately, the cut-off values of this method for pork, chicken and shrimp were 8/5/3 μg/kg, the limits of detection were 1.15/1.28/0.25 μg/kg. The recoveries ranged from 95.5% to 103.0%, with the coefficient of variation from 1.87% to 9.69%. A parallel analysis of 30 samples was confirmed by LC-MS/MS; the results showed a good correlation (R2 > 0.95), indicating the authenticity and reliability of the MIL-88B-NH2-ICA. This work provides a detection strategy with enhanced sensitivity and robustness and simplified sample pretreatment, which has broad application prospects.PMID:37714040 | DOI:10.1016/j.talanta.2023.125190
Source: Talanta - Category: Chemistry Authors: Source Type: research