Glutamine supplementation alleviated aortic atherosclerosis in mice model and in vitro

This study aimed to clarify the role of glutamine in atherosclerosis and its participating mechanism. Forty C57BL/6J mice were divided into wild control (wild Con), ApoE−/− control (ApoE−/− Con), glutamine  + ApoE−/−control (Glut  + ApoE−/− Con), ApoE−/− high fat diet (ApoE−/− HFD), and glutamine  + ApoE−/− HFD (Glut  + ApoE−/− HFD) groups. The degree of atherosclerosis, western blotting, and multiomics were detected at 18  weeks. An in vitro study was also performed. Glutamine treatment significantly decreased the degree of aortic atherosclerosis (p = 0.03).O-GlcNAcylation (O-GlcNAc), IL-1 β, IL-1α, and pyruvate kinase M2 (PKM2) in the ApoE−/− HFD group were significantly higher than those in the ApoE−/− Con group (p <  0.05). These differences were attenuated by glutamine treatment (p <  0.05), and aggravated byO-GlcNA transferase (OGT) overexpression in the in vitro study (p <  0.05). Multiomics showed that the ApoE−/− HFD group had higher levels of oxidative stress regulatory molecules (guanine deaminase [GUAD], xanthine dehydrogenase [XDH]), proinflammatory regulatory molecules (myristic acid and myristoleic acid), and stress granules regulatory molecules (caprin-1 and deoxyribose-phosphate aldolase [DERA]) (p <  0.05). These differences were attenuated by glutamine treatment (p <  0.05). We conclude that glutamine supplementation might alleviate atherosclerosis through downregulation ofO-G...
Source: Proteomics - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research