Impact of 2,4-D and glyphosate-based herbicides on morphofunctional and biochemical markers in Scinax squalirostris tadpoles (Anura, Hylidae)

Chemosphere. 2023 Aug 21:139918. doi: 10.1016/j.chemosphere.2023.139918. Online ahead of print.ABSTRACTUnderstanding the effects of pesticides on non-target organisms is essential to assess the impact of these xenobiotics on the environment, allowing for a more informative and safer usage. The present study sought to evaluate the response of Scinax squalirostris tadpoles when exposed to different concentrations of two herbicides, DEZ® (i.e., dichlorophenoxyacetic acid or 2,4-D) and Roundup® Original (i.e., glyphosate). We collected 140 tadpoles between Gosner's 25 and 34 stages in a preservation area of the South American jelly palm Butia odorata. The animals were separated into eight groups and maintained in aquariums: acclimatization control (17 days), exposure control (24 days), and six exposure groups (7 days), including three concentration groups of each pesticide (4 μg/L, 15 μg/L, and 30 μg/L 2,4-D acid equivalent; 65 μg/L, 250 μg/L, and 500 μg/L glyphosate acid equivalent). Markers of body condition (length, body mass, K and Kn index) and oxidative balance (superoxide dismutase, catalase, glutathione S-transferase, TBARS, and carbonyl proteins) were analyzed. After 24 days (17 days of acclimation plus 7 days of exposure), tadpoles exposed to 15 μg/L of 2,4-D and 65 μg/L of glyphosate grew at higher than expected concentrations. They also had less lipoperoxidation than control tadpoles and higher superoxide dismutase, catalase, and glutathione S-transferase ac...
Source: Chemosphere - Category: Chemistry Authors: Source Type: research