Nano-chitosan/bovine lactoperoxidase and lactoferrin formulation modulates the hepatic deterioration induced by 7,12-dimethylbenz[a]anthracene

This study aimed to evaluate the therapeutic potential of a prepared nano-combination composed of LPO and LF loaded on chitosan nanoparticles (LPO  + LF/CNPs) against oxidative damage in the liver of rats exposed to 7,12-dimethylbenz[a]anthracene (DMBA). Twenty-four male Wistar albino rats (4 months old) were divided into three equal groups and treated orally as follow: (1) rats were given saline and served as control, (2) rats received a single dose (20 mg/kg) of DMBA dissolved in soy oil, and (3) rats intoxicated with a single dose (20 mg/kg) of DMBA, 56 days prior to their oral treatment with the nano-combination (50 mg /kg/day) for consecutive 30 days. Then, blood and liver samples were collected for biochemical determinati ons. Histological and immunohistochemistry examinations of liver tissue were also performed. TEM image of LPO + LF/CNPs showed semi-rounded shape particles with an average size of 336.8 nm and 47.30 mV zeta potential. DMBA intoxication increased serum aspartate aminotransferase (ASAT), alanine aminotransferase (ALAT), and gamma-glutamyl transferase (GGT) activities. DMBA increased also the values of hepatic malondialdehyde (MDA) and nitric oxide (NO), serum nuclear factor κB (NF-κB), caspase-3, interleukin-1beta (IL-1β), and tumor necrosis factor-alpha (TNF- α), and the tumor markers alpha fetoprotein (AFP) and carcino embryonic antigen (CEA), whereas hepatic glutathione (GSH) content and the activities of hepatic glutathione peroxi...
Source: Comparative Clinical Pathology - Category: Pathology Source Type: research