Inhibitory activity of Limosilactobacillus reuteri isolated from camel milk against Helicobacter pylori effects in human gastric epithelial cells

This study aimed to evaluate anti-Helicobacter pylori effects ofLimosilactobacillus reuteri 2892 (L. reuteri 2892) isolated from camel milk in GC cell lines (AGS and MKN). From 15 camel milk samples, 132 microbial strains were isolated. Based on microbial and biochemical analysis, 11 potential probiotic candidates were selected. The potential probiotic candidates were assayed for anti-H. pylori activity, and the strain with the highest anti-H. pylori activity was identified genotypically. Based on 16S rDNA sequencing, the selected strain with the best activity againstH. pylori (inhibition zone  = 15.5 ± 0.8) belonged to theLactobacillus reuteri strain 2892. Cell treatment withH. pylori HC-113 inhibits gene expression of Claudin-4, ZO-1, MUC5AC, and MUC2 in gastric cells, which are attenuated byL. reuteri 2892. The simulative effects ofH. pylori HC-113 on the cell migration and invasion of gastric cells were lost when cells were cotreated withL. reuteri 2892. Cell treatment withH. pylori HC-113 promoted cell death, whereas cotreatment withL. reuteri 2892 markedly decreased necrotic and late apoptotic cells. The present study demonstrates thatL. reuteri 2892 has potent anti-H. pylori effects and thus can be considered as an alternative protective agent against inflammatory effects ofH. pylori in gastric cells.
Source: Biotechnology and Applied Biochemistry - Category: Biochemistry Authors: Tags: ORIGINAL ARTICLE Source Type: research