Mechanical stress induced EndoMT in endothelial cells through PPAR γ downregulation

In this study, elastic silicone chambers were used to simulate the effects of mechanical stretch on ECs under portal hypertension. We found that mechanical stretch decreased PPARγ expression in ECs by blocking the PI3K/AKT/CREB signaling pathway or increasing NEDD4-mediated ubiquitination and degradation of PPARγ. Moreover, PPARγ downregulation triggered Endothelial-to-mesenchymal transition (EndoMT) in ECs under stretch by promoting Smad3 phosphorylation. The PPARγ agonist rosiglitazone mitigated stretch-induced EndoMT in vitro and alleviated EndoMT of the portal vein endothelium in cirrhotic rats.PMID:37468053 | DOI:10.1016/j.cellsig.2023.110812

Source: Cellular Signalling - July 19, 2023 Category: Cytology Authors: Gang Dong Xiaoquan Huang Ye Xu Rongxin Chen Shiyao Chen Source Type: research